Lactate transport by cardiac sarcolemmal vesicles

Abstract

Page C483: T. L. Trosper and K. D. Philipson. “Lactate transport by cardiac sarcolemmal vesicles.” Page C483: the first sentence of paragraph 2 should read: Recent reports of lactate uptake by isolated cardiac myocytes (14) and perfused hearts (2, 16) presented evidence suggesting that lactate traversal of the cardiac sarcolemma is mediated by a carrier specific for monocarboxylic acids. Page C485: Fig. 1 legend should read: l-Lactate uptake into sarcolemmal vesicles as a function of time at room temperature. 1 mM l-lactate in external medium; vesicles loaded with 280 mM sucrose, pH 7.4. External media: 112 mM NaCl, 56 mM sucrose, pH 7.4, with ( closed circles) or without ( open circles) 5 μM monensin; or 280 mM sucrose, pH 7.4 (X) or 5.9 ( open squares). Data points on upper curves are means ± SD of 3 or more experiments. Lower curves are averages of 2 experiments. Inset: l-lactate uptake at short times from NaCl plus monensin, pH 7.4. Page C485: last line of Fig. 3 legend should read: Slope, which is least-squares fit of the data, gives apparent Km, for l-laxtate of 27 mM. Page C489: References 2, 14, and 16 should read: 2. Dennis, S. C., M. C. Cohn, G. J. Anderson, and D. Garfinkel. Kinetic analysis of monocarboxylate uptake into perfused rat hearts. J. Mol. Cell. Cardiol. 17: 987–995, 1985. 14. Kammermeier, H., B. Wein, and W. Graf. Characteristics of lactate transport in isolated cardiac myocytes. Basic Res. Cardiol. 80,Suppl. 1:57–60, 1985. 16. Mann, G. E., B. V. Zlokovic, AND D. L. Yudilevich. Evidence for a lactate transport system in the sarcolemmal membrane of the perfused rabbit heart. Biochim. Biophys. Acta 819: 241–246, 1985.