Electron probe microanalysis of red blood cells. I. Methods and evaluation

Abstract

The concentrations of potassium, sodium, and iron in human and sheep red blood cells were measured with an electron probe. Cells were prepared for analysis by spraying them on pyrolytic graphite supports. The results obtainedwith this spray technique agreed well with values measured on similar cells that were prepared for analysis by freezing, sectioning, and freeze-drying. Higher Na concentrations and lower K concentrations were found to be associated with lower cell volumes in human and high-potassium sheep cells. In low-potassium sheep cells the reverse was found, lower Na and higher K concentrations were associated with lower cell volumes. However, the amounts of iron were found to remain relatively constant in all human cells.