Affiliation:
1. Department of Biomedical Engineering, Virginia Commonwealth University, Richmond, Virginia, United States
Abstract
3D cell culture of OCY454 cells promoted increased differentiation compared with traditional 2D culture. Although Cx43 deficiency did not affect OCY454 differentiation, it resulted in increased signaling, promoting osteoblastogenesis and osteoclastogenesis. Our results suggest that Cx43 deficiency promotes increased bone remodeling in a cell-autonomous manner with minimal changes in osteocyte differentiation. Also, 3D cultures appear better suited to study mechanisms in Cx43-deficient OCY454 osteocytes.
Funder
HHS | National Institutes of Health
National Aeronautics and Space Administration
Publisher
American Physiological Society