Brachyury promotes extracellular matrix synthesis through transcriptional regulation of Smad3 in nucleus pulposus

Author:

Xia Yanzhang12,Wu Yinghui12,Gong Yuhao12,Yue Caichun12,Tao Linfeng3,Xin Tianwen12,Shen Cong4,Zhu Yue5,Shen Minghong6,Shen Jun12ORCID

Affiliation:

1. Department of Orthopedics Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

2. Suzhou Key Laboratory of Orthopedic Medical Engineering, Suzhou University, Suzhou, People’s Republic of China

3. Department of Critical Care Medicine, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

4. State Key Laboratory of Reproductive Medicine, Center for Reproduction and Genetics, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

5. Department of Breast and Thyroid Surgery, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

6. Department of Pathology, The Affiliated Suzhou Hospital of Nanjing Medical University, Suzhou Municipal Hospital, Gusu School, Nanjing Medical University, Suzhou, People’s Republic of China

Abstract

Mechanically, in the nucleus, Bry enhances the transcription of Smad3, leading to increased expression of Smad3 protein levels; in the cytoplasm, elevated substrate levels further lead to an increase in the phosphorylation of Smad3, thereby regulating collagen II and aggrecan expression. Further in vivo experiments provide additional evidence that Bry can alleviate IVDD through this mechanism.

Funder

Key Project of Social Development in Jiangsu--Clinical Frontier Technology

Gusu Health Talent Project of Suzhou

Technology Project of Suzhou Health Commission

Publisher

American Physiological Society

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