MHC-based fiber type and E-C coupling characteristics in mechanically skinned muscle fibers of the rat

Abstract

In this study, we investigated whether the previously established differences between fast- and slow-twitch single skeletal muscle fibers of the rat, in terms of myosin heavy chain (MHC) isoform composition and contractile function, are also detectable in excitation-contraction (E-C) coupling. We compared the contractile responsiveness of electrophoretically typed, mechanically skinned single fibers from the soleus (Sol), the extensor digitorum longus (EDL), and the white region of the sternomastoid (SM) muscle to t-system depolarization-induced activation. The quantitative parameters assessed were the amplitude of the maximum depolarization-induced force response (DIFRmax; normalized to the maximum Ca2+-activated force in that fiber) and the number of responses elicited until the force declined by 75% of DIFRmax (R-D75%). The mean DIFRmaxvalues for type IIB EDL and type IIB SM fibers were not statistically different, and both were greater than the mean DIFRmax for type I Sol fibers. The mean R-D75% for type IIB EDL fibers was greater than that for type I Sol fibers as well as type IIB SM fibers. These data suggest that E-C coupling characteristics of mechanically skinned rat single muscle fibers are related to MHC-based fiber type and the muscle of origin.