Determinants of atrial natriuretic peptide secretion in cultured atrial myocytes

Author:

Iida H.1,Page E.1

Affiliation:

1. Department of Medicine, University of Chicago, Illinois 60637.

Abstract

Atrial natriuretic peptide (ANP) secretion by atrial myocytes in 7- to 8-day primary cultures from adult rats was measured by radioimmunoassay under conditions designed to separate primary effects on secretion from effects caused by contractions. Abolishing contraction with 10 microM tetrodotoxin significantly reduced ANP accumulated in 2 h. Raising external Ca2+ concentration from 0.2 to 1.2 mM in the presence of tetrodotoxin did not increase ANP secretion. Substantial ANP secretion persisted in a nominally Ca2+-free medium containing 10 mM ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid (EGTA) and was not diminished by 100 microM ryanodine. In the presence of EGTA, 100 nM 12-O-tetradecanoylphorbol 13-acetate (TPA) significantly increased ANP secretion; this increment was unaffected by 100 microM ryanodine. These experiments suggest 1) that in absence of contractions, ANP secretion requires neither transplasmalemmal Ca2+ influx nor ryanodine-inhibitable Ca2+ release by sarcoplasmic reticulum (SR) and 2) that TPA stimulates ANP secretion without requiring Ca influx or ryanodine-inhibitable SR Ca release.

Publisher

American Physiological Society

Subject

Cell Biology,Physiology

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1. Stretch-activated non-selective cation channel: A causal link between mechanical stretch and atrial natriuretic peptide secretion;Progress in Biophysics and Molecular Biology;2008-09

2. The Role of Endothelin in Mediating Ischemia/Hypoxia-Induced Atrial Natriuretic Peptide Release;Journal of Cardiovascular Pharmacology;2004-02

3. Distinct roles for L- and T-type Ca2+ channels in regulation of atrial ANP release;American Journal of Physiology-Heart and Circulatory Physiology;2000-12-01

4. Role of cell contractions in cAMP-induced cardiomyocyte atrial natriuretic peptide release;American Journal of Physiology-Heart and Circulatory Physiology;2000-01-01

5. Potentiation of stretch-induced atrial natriuretic peptide secretion by intracellular acidosis;American Journal of Physiology-Heart and Circulatory Physiology;1999-07-01

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