Expression of TWIK-1, a novel weakly inward rectifying potassium channel in rat kidney

Author:

Cluzeaud F.1,Reyes R.2,Escoubet B.3,Fay M.1,Lazdunski M.2,Bonvalet J. P.1,Lesage F.2,Farman N.1

Affiliation:

1. Unité 478 and

2. Institut de Pharmacologie Moléculaire et Cellulaire, Centre National de la Recherche Scientifique, 06560 Valbonne, France

3. Unité 426, Institut National de la Santé et de la Recherche Médicale, Faculté de Médecine X. Bichat, F-75870 Paris cedex 18; and

Abstract

Several K+ conductances have been identified in the kidney, with specific properties and localization in distinct cell types and membrane domains. On the other hand, several K+ channels have been characterized at the molecular level. By immunolocalization, we show that a new inward rectifying K+channel, TWIK-1, is specifically expressed in distinct tubular segments and cell types of the rat kidney. In the proximal tubule, TWIK-1 prevails in the initial portions (convoluted part), where it is restricted to the apical (brush-border) membrane. In the collecting duct, immunofluorescence was intracellular or confined to the apical membrane and restricted to intercalated cells, i.e., in cells lacking aquaporin-2, as shown by double immunofluorescence. TWIK was also expressed in medullary and cortical parts of the thick limb of the loop of Henle, identified with an anti-Tamm-Horsfall protein antibody (double immunofluorescence). The intensity of TWIK-1 immunolabeling was unchanged in rats fed a low-Na+ or a low-K+ diet. Because TWIK-1 shares common properties with the low-conductance apical K+ channel of the collecting duct, we propose that it could play a role in K+ secretion, complementary to ROMK, another recently characterized K+ channel located in principal cells of the cortical collecting duct and in the loop of Henle.

Publisher

American Physiological Society

Subject

Cell Biology,Physiology

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