Isometric, shortening, and lengthening contractions of muscle fiber segments from adult and old mice

Abstract

In old animals, skeletal muscle force decreases during both isometric and shortening contractions. In contrast, force during lengthening appears to be unaffected by aging. We hypothesized that with aging single permeabilized muscle fibers would demonstrate the same impairments in force as are observed for whole muscles. For single permeabilized fibers from extensor digitorum longus muscles of adult and old mice, forces were measured during isometric, shortening, and lengthening contractions performed at 15 degrees C. Maximum isometric forces normalized for fiber area were not different for fibers from adult and old mice. During submaximal isometric contractions a decreased calcium sensitivity resulted in lower forces for fibers from old compared with adult mice. In contrast to a lack of difference in forces developed by fibers from old and adult mice during shortening contractions, during lengthening contractions fibers from old mice developed forces approximately 30% higher than those of adult mice. We conclude that the impairments in force of whole muscles with aging are not the result of impairments in intrinsic force-generating capacity of cross bridges, but changes do occur in single permeabilized muscle fibers of old mice that result in higher forces during stretch.