Author:
van den Broek Nicole M. A.,De Feyter Henk M. M. L.,Graaf Larry de,Nicolay Klaas,Prompers Jeanine J.
Abstract
31P magnetic resonance spectroscopy provides the possibility of obtaining bioenergetic data during skeletal muscle exercise and recovery. The time constant of phosphocreatine (PCr) recovery (τPCr) has been used as a measure of mitochondrial function. However, cytosolic pH has a strong influence on the kinetics of PCr recovery, and it has been suggested that τPCr should be normalized for end-exercise pH. A general correction can only be applied if there are no intersubject differences in the pH dependence of τPCr. We investigated the pH dependence of τPCr on a subject-by-subject basis. Furthermore, we determined the kinetics of proton efflux at the start of recovery. Intracellular acidosis slowed PCr recovery, and the pH dependence of τPCr differed among subjects, ranging from −33.0 to −75.3 s/pH unit. The slope of the relation between τPCr and end-exercise pH was positively correlated with both the proton efflux rate and the apparent proton efflux rate constant, indicating that subjects with a smaller pH dependence of τPCr have a higher proton efflux rate. Our study implies that simply correcting τPCr for end-exercise pH is not adequate, in particular when comparing patients and control subjects, because certain disorders are characterized by altered proton efflux from muscle fibers.
Publisher
American Physiological Society
Cited by
77 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献