Multiomic analysis defines the first microRNA atlas across all small intestinal epithelial lineages and reveals novel markers of almost all major cell types-Reference-Cited by-同舟云学术

Multiomic analysis defines the first microRNA atlas across all small intestinal epithelial lineages and reveals novel markers of almost all major cell types

Published:2021-12-01 Issue:6 Volume:321 Page:G668-G681
ISSN:0193-1857
Container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology
language:en
Short-container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology

Author:

Shanahan Michael T.¹,Kanke Matt¹,Oyesola Oyebola O.²,Hung Yu-Han¹,Koch-Laskowski Kieran¹,Singh Ajeet P.¹,Peck Bailey C. E.³,Biraud Mandy⁴,Sheahan Breanna⁴,Cortes Josca E.⁴,Gong Huiyu⁵⁶,Sahoo Dipak K.⁷,Cubitt Rebecca¹,Kurpios Natasza A.⁸,Mochel Jonathan P.⁷,Allenspach Karin⁷,McElroy Steven J.⁵⁶^ORCID,Ding Shengli⁹,von Moltke Jakob²,Dekaney Christopher M.⁴,Tait-Wojno Elia D.²,Sethupathy Praveen¹^ORCID

Affiliation:

1. Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York

2. Department of Immunology, University of Washington, Seattle, Washington

3. Department of Surgery, University of Michigan, Ann Arbor, Michigan

4. Department of Molecular Biomedical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina

5. Department of Pediatrics, University of Iowa, Iowa City, Iowa

6. Department of Microbiology and Immunology, University of Iowa, Iowa City, Iowa

7. Department of Biomedical Sciences, College of Veterinary Medicine, Iowa State University, Ames, Iowa

8. Department of Molecular Medicine, College of Veterinary Medicine, Cornell University, Ithaca, New York

9. Department of Biomedical Engineering, Pratt School of Engineering, Duke University, Durham, North Carolina

Abstract

In this study, first, microRNA atlas (and searchable web server) across all major small intestinal epithelial cell types is presented. We have demonstrated microRNAs that uniquely mark several lineages, including enteroendocrine and tuft. Identification of a key marker of mouse secretory progenitor cells, miR-672, which we show is deleted in humans. We have used several in vivo models to establish miR-152 as a specific marker of Paneth cells, which are highly understudied in terms of microRNAs.

Funder

American Diabetes Association Research Foundation

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpgi.00222.2021

Reference57 articles.

1. MicroRNAs Control Intestinal Epithelial Differentiation, Architecture, and Barrier Function

2. Functional Transcriptomics in Diverse Intestinal Epithelial Cell Types Reveals Robust MicroRNA Sensitivity in Intestinal Stem Cells to Microbial Status

3. Enteroendocrine Progenitor Cell–Enriched miR-7 Regulates Intestinal Epithelial Proliferation in an Xiap-Dependent Manner