Adult enteric Dclk1-positive glial and neuronal cells reveal distinct responses to acute intestinal injury

Author:

Middelhoff Moritz12ORCID,Valenti Giovanni1,Tomassoni Lorenzo3,Ochiai Yosuke1,Belin Bryana1,Takahashi Ryota4,Malagola Ermanno1,Nienhüser Henrik5,Finlayson Michael3,Hayakawa Yoku4ORCID,Zamechek Leah B.1,Renz Bernhard W.6,Westphalen C. Benedikt7,Quante Michael8,Margolis Kara G.9ORCID,Sims Peter A.310,Laise Pasquale311ORCID,Califano Andrea3,Rao Meenakshi12ORCID,Gershon Michael D.13ORCID,Wang Timothy C.1ORCID

Affiliation:

1. Division of Digestive and Liver Diseases, Department of Medicine, Columbia University College of Physicians and Surgeons, New York, New York

2. Klinik und Poliklinik für Innere Medizin II, Klinikum rechts der Isar, Technische Universität München, Munich, Germany

3. Department of Systems Biology, Columbia University College of Physicians and Surgeons, New York, New York

4. Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan

5. Department of General, Visceral and Transplantation Surgery, Heidelberg University Hospital, Heidelberg, Germany

6. Department of General, Visceral and Transplantation Surgery, Hospital of the University of Munich, Munich, Germany

7. Department of Internal Medicine, Comprehensive Cancer Center, Hospital of the University of Munich, Munich, Germany

8. Klinik für Innere Medizin II, Gastrointestinale Onkologie, Universitätsklinikum Freiburg, Freiburg, Germany

9. Department of Pediatrics, Columbia University College of Physicians and Surgeons, New York, New York

10. Department of Biochemistry and Molecular Biophysics, Columbia University College of Physicians and Surgeons, New York, New York

11. DarwinHealth Inc., New York, New York

12. Division of Gastroenterology, Hepatology and Nutrition, Boston Children´s Hospital, Harvard Medical School, Boston, Massachusetts

13. Department of Pathology and Cell Biology, Columbia University College of Physicians and Surgeons, New York, New York

Abstract

The kinase DCLK1 identifies glial-like and neuronal cell types in adult murine enteric ganglia, which resist acute injury-mediated cell death yet differ in their cellular response to injury. Interestingly, Dclk1-labeled glial-like cells show prominent transcriptional changes in response to injury and harbor features reminiscent of previously described enteric neural precursor cells. Our data thus add to recently emerging evidence of reserve cellular plasticity in the adult enteric nervous system.

Funder

Deutsche Krebshilfe

HHS | NIH | National Cancer Institute

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

HHS | NIH | NIH Office of the Director

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

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