Simultaneous whole-cell patch-clamp and calcium imaging on myenteric neurons-Reference-Cited by-同舟云学术

Simultaneous whole-cell patch-clamp and calcium imaging on myenteric neurons

Published:2022-10-01 Issue:4 Volume:323 Page:G341-G347
ISSN:0193-1857
Container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology
language:en
Short-container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology

Author:

Li Zhiling¹²,Boesmans Werend³⁴^ORCID,Kazwiny Youcef¹,Hao Marlene M.¹⁵^ORCID,Vanden Berghe Pieter¹⁶^ORCID

Affiliation:

1. Laboratory for Enteric Neuroscience, Translational Research Center for Gastrointestinal Disorders, ChroMeta, Katholieke Universiteit Leuven, Leuven, Belgium

2. The Brain Cognition and Brain Disease Institute, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People’s Republic of China

3. Biomedical Research Institute, Hasselt University, Hasselt, Belgium

4. Department of Pathology, GROW-School for Oncology and Reproduction, Maastricht University Medical Centre, Maastricht, The Netherlands

5. Department of Anatomy and Physiology, The University of Melbourne, Parkville, Victoria, Australia

6. Leuven Brain Institute, Katholieke Universiteit Leuven, Leuven, Belgium

Abstract

Live calcium imaging is an important tool for investigating enteric nervous system (ENS) function. Previous studies have shown that multiple action potentials are needed to generate a calcium response in S-neurons, which has important implications for the interpretation of calcium imaging data. Here, we show that in mouse myenteric neurons, calcium transients are elicited by single action potentials in both AH- and S-neurons. In addition, nicotinic acetylcholine receptor stimulation can be used to distinguish between these two classes.

Funder

Department of Education and Training | Australian Research Council

Department of Health | National Health and Medical Research Council

Fonds Wetenschappelijk Onderzoek

Hercules Foundation

NWO | Nationaal Regieorgaan Onderwijsonderzoek

China Scholarship Council

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

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