Affiliation:
1. Departments of Anatomy and Cell Biology and
2. Center for Neurobiology and Behavior, Columbia University, College of Physicians and Surgeons, New York, New York 10032.
Abstract
The distribution and function of the 5-hydroxytryptamine (5-HT2A) receptor were investigated in the intestines of wild-type (5-HT2A +/+) and knockout (5-HT2A−/−) mice. In 5-HT2A +/+ mice, rats, and guinea pigs, 5-HT2A receptor immunoreactivity was found on circular and longitudinal smooth muscle cells, neurons, enterocytes, and Paneth cells. Muscular 5-HT2A receptors were concentrated in caveolae; neuronal 5-HT2A receptors were found intracellularly and on the plasma membranes of nerve cell bodies and axons. Neuronal 5-HT2A immunoreactivity was detected as early as E14 in ganglia, intravillus nerves, and the deep muscle plexus. The 5-HT2A −/− colon did not express 5-HT2A receptors and did not contract in response to exogenous 5-HT. 5-HT2A −/− enterocytes were smaller, Paneth cells fewer, and muscle layers thinner (and showed degeneration) compared with those of 5-HT2A +/+ littermates. The 5-HT2A receptor may thus be required for the maintenance and/or development of enteric neuroeffectors and other enteric functions, although gastrointestinal and colonic transit times in 5-HT2A −/− and +/+ mice did not differ significantly.
Publisher
American Physiological Society
Subject
Physiology (medical),Gastroenterology,Hepatology,Physiology
Cited by
100 articles.
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