IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles-Reference-Cited by-同舟云学术

IMP1/IGF2BP1 in human colorectal cancer extracellular vesicles

Published:2022-12-01 Issue:6 Volume:323 Page:G571-G585
ISSN:0193-1857
Container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology
language:en
Short-container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology

Author:

Kuhn Madeline¹,Zhang Yang¹,Favate John²,Morita Mayu³,Blucher Aurora⁴,Das Sukanya²,Liang Shun²,Preet Ranjan⁵,Parham Louis R.⁶,Williams Kathy N.⁷,Molugu Sudheer⁸,Armstrong Randall J.⁴⁹,Zhang Wei¹⁰,Yang Jiegang¹⁰,Hamilton Kathryn E.⁶^ORCID,Dixon Dan A.⁵,Mills Gordon⁴,Morgan Terry K.³⁹,Shah Premal²,Andres Sarah F.¹^ORCID

Affiliation:

1. Pediatric Gastroenterology Division, Department of Pediatrics, School of Medicine, Oregon Health and Science University, Portland, Oregon

2. Department of Genetics, Rutgers University, Piscataway, New Jersey

3. Department of Pathology, Oregon Health and Science University, Portland, Oregon

4. Division of Oncological Sciences, Knight Cancer Institute, Oregon Health and Science University, Portland, Oregon

5. Department of Molecular Biosciences, University of Kansas Cancer Center, University of Kansas, Lawrence, Kansas

6. Division of Gastroenterology Hepatology and Nutrition, Children’s Hospital of Philadelphia, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania

7. Division of Gastroenterology, Department of Medicine, Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania

8. Electron Microscopy Resource Lab, University of Pennsylvania Perelman School of Medicine, Philadelphia, Pennsylvania

9. Cancer Early Detection Advanced Research, Oregon Health and Science University, Portland, Oregon

10. Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania

Abstract

This work demonstrates that the RNA binding protein IGF2BP1/IMP1 alters the transcript profile of colorectal cancer cell (CRC) mRNAs from extracellular vesicle (EV) pathways. IMP1 does not alter EV production or secretion in vitro or in vivo, but rather enters CRC cells where it may further impact EV cargo. Our work shows that IMP1 has the ability to shape EV cargo in human CRC, which could serve as a diagnostic/prognostic circulating tumor biomarker.

Funder

AGA Research Foundation (American Gastroenterological Association

American Cancer Society

HHS | NIH | NCI | Basic Research Laboratory

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

HHS | NIH | National Institute of General Medical Sciences

Lustgarten Foundation

NSF | BIO | Division of Biological Infrastructure

OHSU | Knight Cancer Institute, Oregon Health and Science University

Prevent Cancer Foundation

School of Medicine, Oregon Health and Science University

Publisher