Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, βPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice-Reference-Cited by-同舟云学术

Targeted intestinal deletion of Rho guanine nucleotide exchange factor 7, βPIX, impairs enterocyte proliferation, villus maturation, and mucosal defenses in mice

Published:2021-04-01 Issue:4 Volume:320 Page:G627-G643
ISSN:0193-1857
Container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology
language:en
Short-container-title:American Journal of Physiology-Gastrointestinal and Liver Physiology

Author:

Cheng Kunrong¹²,Larabee Shannon M.³,Tolaymat Mazen²,Hanscom Marie²,Shang Aaron C.²,Schledwitz Alyssa²,Hu Shien¹²,Drachenberg Cinthia B.⁴,Zhan Min⁵,Chahdi Ahmed²,Raufman Jean-Pierre¹²⁶⁷

Affiliation:

1. Veterans Affairs Maryland Healthcare System, Baltimore, Maryland

2. Department of Medicine, Division of Gastroenterology and Hepatology, University of Maryland School of Medicine, Baltimore, Maryland

3. Department of Surgery, University of Maryland School of Medicine, Baltimore, Maryland

4. Department of Pathology, University of Maryland School of Medicine, Baltimore, Maryland

5. Department of Epidemiology and Public Health, University of Maryland School of Medicine, Baltimore, Maryland

6. Marlene and Stewart Greenebaum Cancer Center, University of Maryland School of Medicine, Baltimore, Maryland

7. Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine, Baltimore, Maryland

Abstract

To explore the role of βPix, a guanine nucleotide exchange factor encoded by ARHGEF7, in intestinal development and physiology, we created mice with intestinal epithelial cell Arhgef7/βPix deficiency. We found βPix essential for normal small intestinal epithelial cell proliferation, villus development, and mucosal resistance to injury. Moreover, Rho kinase signaling mediated developmental arrest observed in enteroids derived from βPix-deficient small intestinal crypts. Our studies provide insights into the role Arhgef7/βPix plays in intestinal epithelial homeostasis.

Funder

HHS | National Institutes of Health

U.S. Department of Veterans Affairs

Publisher

American Physiological Society

Subject

Physiology (medical),Gastroenterology,Hepatology,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpgi.00415.2020

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