Effect of Adenosine on Phosphofructokinase Activity of Preserved Blood

Abstract

Adenosine in concentrations of 5–20 mm prevents the progressive decrease in activity of the erythrocyte glycolytic enzyme phosphofructokinase in blood preserved in acid citrate-dextrose at 4°. In hemolysates the protective effect of adenosine is also exhibited by its metabolic derivatives inosine, ribose-5-phosphate and adenosine triphosphate. The beneficial effects of adenosine on in vivo survival of stored red cells, glycolysis and PFK activity suggests that the integrity of this enzyme may influence the keeping qualities of ACD preserved blood.