Affiliation:
1. Departments of Biochemistry, Columbia University College of Physicians and Surgeons; and Walter Reed Army Institute of Research, Washington, D. C.
Abstract
The use of the isolated perfused rat liver for quantitative biochemical studies of liver metabolism over an experimental period of as long as 4 hours is demonstrated. Respiration rates responded immediately to the addition of substrates and the liver could be restimulated by a second dose of substrate, respiration rates reflecting the nature of the substrate. Net amino nitrogen uptake was consistently exceeded by urea nitrogen production, the latter comparing favorably with in vivo rates. Fructose was rapidly removed from the perfusate by the liver, and the liver efficiently removed lactic acid formed by erythrocyte glycolysis. Ketone body production was continuous and within normal in vivo rates. Bile production declined gradually over a 4-hour period.
Publisher
American Physiological Society
Cited by
22 articles.
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