Relation of glutaminase I activity to glutamic acid concentration in the rat kidney

Abstract

Injection of sodium glutamate had no significant effect on renal glutaminase I activity in untreated rats but suppressed the rise in enzyme activity which follows repeated ammonium chloride administration. Injection of glutamine produced a small rise in enzyme activity in untreated rats but suppressed the enzyme response in ammonium chloride-treated animals. Injection of glycine had no significant effect on enzyme activity in treated or untreated rats. A significant fall in renal glutamic acid concentration was observed 2 hours after the administration of ammonium chloride. These results suggest that there is a relation between the concentration of glutamic acid and the synthesis of glutaminase I in the kidney of the rat.