Affiliation:
1. Divisions of Endocrinology and Pediatric Cardiology, Departments of Medicine and Pediatrics, North Shore University Hospital/New York University School of Medicine, Manhasset, New York 11030
Abstract
In a rat model of acute myocardial infarction (MI) produced by coronary artery ligation, thyroid hormone metabolism was altered with significant reductions (54%) in serum triiodo-l-thyronine (T3), the cellular active hormone metabolite. T3 has profound effects on the heart; therefore, rats were treated with T3 after acute MI for 2 or 3 wk, at either replacement or elevated doses, to determine whether cardiac function and gene expression could be normalized. Acute MI resulted in a 50% ( P < 0.001) decrease in percent ejection fraction (%EF) with a 32–35% increase ( P < 0.01) in compensatory left ventricle (LV) hypertrophy. Treatment of the MI animals with either replacement or elevated doses of T3 significantly increased %EF to 64 and 73% of control, respectively. Expression levels of several T3-responsive genes were altered in the hypertrophied LV after MI, including significant decreases in α-myosin heavy chain (MHC), sarcoplasmic reticulum calcium-activated ATPase (SERCA2), and Kv1.5 mRNA, whereas β-MHC and phospholamban (PLB) mRNA were significantly increased. Normalization of serum T3 did not restore expression of all T3-regulated genes, indicating altered T3 responsiveness in the postinfarcted myocardium. Although β-MHC and Kv1.5 mRNA content was returned to control levels, α-MHC and SERCA2 were unresponsive to T3 at replacement doses, and only at higher doses of T3 was α-MHC mRNA returned to control values. The present study showed that acute MI in the rat was associated with a fall in serum T3 levels, LV dysfunction, and altered expression of T3-responsive genes and that T3 treatment significantly improved cardiac function, with normalization of some, but not all, of the changes in gene expression.
Publisher
American Physiological Society
Subject
Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism
Cited by
106 articles.
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