A new method for the study of chylomicron kinetics in vivo

Author:

Park Yongsoon1,Grellner Wayne J.1,Harris William S.1,Miles John M.1

Affiliation:

1. Department of Medicine, University of Missouri-Kansas City, and Cardiovascular Research Department, Mid America Heart Institute, St. Luke's Hospital, Kansas City, Missouri 64111

Abstract

Our understanding of the metabolism of chylomicrons, the lipoprotein that transports dietary fat from the intestine to peripheral tissues, is incomplete. The present studies were conducted to determine whether a labeled intravenous lipid emulsion could be used to estimate chylomicron triglyceride (TG) rate of appearance (Ra) and thereby quantify the rate of intestinal fat absorption. After an overnight fast, healthy volunteers ( n = 6) sipped a3H-labeled drink over 6.5 h at a rate of 175 mg fat · kg−1 · h−1. Beginning at hour 5, an HPLC-purified, 14C-labeled lipid emulsion was infused intravenously for 90 min. During the study, plasma total and chylomicron TG concentrations increased from 100 ± 21 to 237 ± 40 mg/dl and from undetectable to steady-state levels of 35 ± 13 mg/dl, respectively. After a minor correction for VLDL contamination, tracer-determined chylomicron TG Ra was 175 ± 30 mg · kg−1 · h−1, equal to the presumed ingestion rate. In summary, a radiolabeled intravenous lipid emulsion is able to accurately estimate chylomicron TG Raand therefore can be used to measure in vivo fat absorption rates.

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism

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