Evaluation of glucagon-like peptide-1 receptor expression in nondiabetic and diabetic atherosclerotic mice using PET tracer 68Ga-NODAGA-exendin-4-Reference-Cited by-同舟云学术

Evaluation of glucagon-like peptide-1 receptor expression in nondiabetic and diabetic atherosclerotic mice using PET tracer 68Ga-NODAGA-exendin-4

Published:2021-05-01 Issue:5 Volume:320 Page:E989-E998
ISSN:0193-1849
Container-title:American Journal of Physiology-Endocrinology and Metabolism
language:en
Short-container-title:American Journal of Physiology-Endocrinology and Metabolism

Author:

Ståhle Mia¹^ORCID,Hellberg Sanna¹,Virta Jenni¹,Liljenbäck Heidi¹²,Metsälä Olli¹,Li Xiang-Guo¹³,Jauhiainen Matti⁴,Saukko Pekka⁵,Ylä-Herttuala Seppo⁶,Nuutila Pirjo¹⁷^ORCID,Knuuti Juhani¹⁸,Saraste Antti¹⁸⁹,Roivainen Anne¹²^ORCID

Affiliation:

1. Turku PET Centre, University of Turku, Turku, Finland

2. Turku Center for Disease Modeling, University of Turku, Turku, Finland

3. Turku PET Centre, Åbo Akademi University, Turku, Finland

4. Minerva Foundation Institute for Medical Research and Genomics and Biomarkers Unit, National Institute for Health and Welfare, Biomedicum, Helsinki, Finland

5. Department of Pathology and Forensic Medicine, University of Turku, Turku, Finland

6. A.I. Virtanen Institute for Molecular Sciences, University of Eastern Finland, Kuopio, Finland

7. Department of Endocrinology, Turku University Hospital, Turku, Finland

8. Turku PET Centre, Turku University Hospital, Turku, Finland

9. Heart Center, Turku University Hospital, Turku, Finland

Abstract

Cardiovascular effects of glucagon-like peptide-1 receptor (GLP-1R) agonist therapies are potentially mediated by anti-inflammatory effects on atherosclerosis. Our study demonstrates that 68Ga-NODAGA-exendin-4, a radioligand specifically targeting GLP-1R, detects GLP-1R expression in inflamed atherosclerotic lesions in nondiabetic and diabetic hypercholesterolemic mice. Immunofluorescence staining suggests that GLP-1R is primarily localized in M2 macrophages in lesions. This study describes a new potential tool that may have translational relevance for studies of pharmacological modification of GLP-1R signaling in atherosclerosis.

Funder

Sigrid Juselius Foundation

Instrumentarium Science Foundation

Jalmari and Rauha Ahokas Foundation

Finnish Foundation for Cardiovascular Research

Ida Montinin Säätiö

Suomen Kulttuurirahasto

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism

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