Proteome analysis of human adipocytes identifies depot-specific heterogeneity at metabolic control points

Author:

Raajendiran Arthe123,Krisp Christoph4,Souza David P. De5,Ooi Geraldine6,Burton Paul R.6,Taylor Renea A.27,Molloy Mark P.4,Watt Matthew J.1

Affiliation:

1. Department of Anatomy and Physiology, University of Melbourne, Melbourne, Victoria, Australia

2. Department of Physiology, Monash University, Clayton, Victoria, Australia

3. Metabolism, Diabetes and Obesity Program, Monash Biomedicine Discovery Institute, University of Melbourne, Melbourne, Victoria, Australia

4. Australian Proteome Analysis Facility, Macquarie University, New South Wales, Australia

5. Metabolomics Australia, Bio21 Institute of Molecular Science and Biotechnology, University of Melbourne, Parkville, Victoria, Australia

6. Faculty of Medicine, Nursing and Health Sciences, Centre for Obesity Research and Education, Monash University, Melbourne, Victoria, Australia

7. Cancer Research Division, Peter MacCallum Cancer Centre, University of Melbourne, Melbourne, Victoria, Australia

Abstract

Adipocyte metabolism varies depending on anatomical location and the adipocyte protein composition may orchestrate this heterogeneity. We used SWATH proteomics in patient-matched human upper- (visceral and subcutaneous) and lower-body (glutealfemoral) adipocytes and detected 4,220 proteins and distinguishable regional proteomes. Upper-body adipocyte proteins were associated with glycolysis, de novo lipogenesis, mitochondrial dysfunction, and oxidative stress, whereas lower-body adipocyte proteins were associated with enhanced PPARα activation, fatty acid, and BCAA oxidation, TCA cycle flux, and oxidative phosphorylation.

Funder

Department of Health, Australian Government | National Health and Medical Research Council

Victorian Cancer Agency

Publisher

American Physiological Society

Subject

Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism

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