Glutamate transport asymmetry in renal glutamine metabolism

Abstract

d-Glutamate (Glu) was previously shown to block l-Glu uptake and accelerate glutaminase flux in cultured kidney cells [Welbourne, T. C., and D. Chevalier. Am. J. Physiol. 272 ( Endocrinol. Metab. 35): E367–E370, 1997]. To test whether d-Glu would be taken up by the intact functioning kidney and effect the same response in vivo, male Sprague-Dawley rats were infused withd-Glu (2.6 μmol/min), and renal uptake of d- andl-Glu was determined from chemical and radiolabeled arteriovenous Glu concentration differences times renal plasma flow. The amount removed was then compared with that amount filtered to obtain the antiluminal contribution. In the controls, l-Glu uptake measured as net removal was 33% of the arteriall-Glu load and not different from that filtered, 27%; however, the unidirectional uptake was actually 58% of the arterial load, indicating that antiluminal uptake contributes at least half to the overall Glu consumption. Surprisingly, the kidneys showed a more avid removal ofd-Glu, removing 73% of the arterial load, indicating uptake predominantly across the antiluminal cell surface. Furthermore, uptake ofd-Glu was associated with a 55% reduction in l-Glu uptake, with the residual amount taken up equivalent to that filtered;d-Glu did not increase the excretion of the l-isomer. However, elevating plasma l-Glu concentration reduced uptake of thed-isomer, suggesting a shared antiluminal transporter. Thus there is an apparent asymmetrical distribution of the d-Glu transporter. Under these conditions, kidney cortexl-Glu content decreased 44%, whereas net glutamine (Gln) uptake increased sevenfold (170 ± 89 to 1,311 ± 219 nmol/min, P < 0.01) and unidirectional uptake nearly threefold (393 ± 121 to 1,168 ± 161 nmol/min, P < 0.05); this large Gln consumption was paralleled by an increase in ammonium production so that the ratio of production to consumption approaches 2, consistent with accelerated Gln deamidation and subsequent Glu deamination. These results point to a functional asymmetry (antiluminal vs. luminal) for Glu transporter activity, which potentially plays an important role in modulating Gln metabolism and renal function.