Sexual dimorphism in the growth response of entire and gonadectomized rats to clenbuterol

Abstract

This study compared the anabolic effects of clenbuterol in male and female rats and determined the relative contribution of testicular and ovarian hormones to any observed gender difference. Seventy-two 12-wk-old rats were used in a 2 x 2 x 2 factorial design in which animals were either male or female, entire or gonadectomized at 3 wk of age, and fed either a control diet or a diet containing 4 mg clenbuterol/kg feed for 8 days. Compared with entire male rats, entire females gained 64% less weight, had lighter carcasses (-36%) and gastrocnemius muscles (-62%), and had higher plasma concentrations of the catabolic hormone corticosterone (P < 0.05). Castration had a negative effect on growth in male rats, and ovariectomy had a positive effect in females, but there was still a gender difference in body weight between gonadectomized males and females, which amounted to 34% of the gender difference observed in intact rats. The density of beta 2-adrenoceptors in skeletal muscle was not different between males and females, nor was it affected by gonadectomy. Clenbuterol increased both weight gain and gastrocnemius muscle weight, with the latter response in entire and castrated male rats (+ 1.31 and + 1.17 g) being more than double that seen in entire and ovariectomized females (+ 0.58 and + 0.55 g). The downregulation response of beta 2-adrenoceptors in this muscle was remarkably consistent in all treated groups (-50% to -53%).(ABSTRACT TRUNCATED AT 250 WORDS)