Affiliation:
1. Departments of Medicine,
2. Pharmacology,
3. Mouse Metabolic Physiology Center, Vanderbilt University School of Medicine, Nashville, Tennessee 37232
4. Molecular Physiology and Biophysics, and
5. Radiology,
Abstract
Isotopic techniques were used to test the hypothesis that exercise and nitric oxide synthase (NOS) inhibition have distinct effects on tissue-specific fatty acid and glucose uptakes in a conscious, chronically catheterized mouse model. Uptakes were measured using the radioactive tracers125I-labeled β-methyl- p-iodophenylpentadecanoic acid (BMIPP) and deoxy-[2-3H]glucose (DG) during treadmill exercise with and without inhibition of NOS. [125I]BMIPP uptake at rest differed substantially among tissues with the highest levels in heart. With exercise, [125I]BMIPP uptake increased in both heart and skeletal muscles. In sedentary mice, NOS inhibition induced by nitro-l-arginine methyl ester (l-NAME) feeding increased heart and soleus [125I]BMIPP uptake. In contrast, exercise, but not l-NAME feeding, resulted in increased heart and skeletal muscle [2-3H]DG uptake. Significant interactions were not observed in the effects of combined exercise and l-NAME feeding on [125I]BMIPP and [2-3H]DG uptakes. In the conscious mouse, exercise and NOS inhibition produce distinct patterns of tissue-specific fatty acid and glucose uptake; NOS is not required for important components of exercise-associated metabolic signaling, or other mechanisms compensate for the absence of this regulatory mechanism.
Publisher
American Physiological Society
Subject
Physiology (medical),Physiology,Endocrinology, Diabetes and Metabolism
Cited by
51 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献