Effects of calcitonin gene-related peptide and efferent nerve stimulation on afferent transmission in the lateral line organ

Abstract

1. Calcitonin gene-related peptide (CGRP) is a 37-amino acid peptide immunolocalized in efferent fibers innervating hair-cell organs, including the lateral line organ of Xenopus laevis. CGRP, applied in nanomolar concentrations, increased the spontaneous discharge rate in afferent fibers innervating hair cells of the lateral line organ. 2. The increase in spontaneous discharge rate with application of CGRP was associated with an increase in the rate of occurrence of spontaneous excitatory postsynaptic potentials (EPSPs) and with little change in the amplitude of the EPSPs. 3. Prolonged (several hundred seconds) application of CGRP produced an increase in afferent fiber discharge rate that returned to control values in the continued presence of the peptide. 4. Efferent fibers were electrically stimulated to look for a non-cholinergic effect on spontaneous afferent discharge that might be attributed to CGRP. Electrical stimulation of the efferent fibers produced a rapid (100 ms) suppression of discharge rate followed by a rapid (100 ms) increase in discharge rate. However, both the rapid suppression and rapid excitation were likely to be mediated by the release of acetylcholine, because they were blocked by the application of the cholinergic blocking agents curare and atropine as well as by strychnine. 5. In almost one-half of the preparations examined, electrical stimulation of efferent fibers also produced a slowly developing increase in afferent discharge that could persist for several minutes after termination of the shocks. 6. This slow excitation by efferent stimulation was not blocked by concentrations of curare that blocked the rapid effects of efferent stimulation. Thus the slow effect is likely to be mediated by a receptor different from that for the rapid cholinergic effects. One possibility is that the excitation is mediated by the release of CGRP from the efferent nerve fibers.