Sound localization in anurans. I. Evidence of binaural interaction in dorsal medullary nucleus of bullfrogs (Rana catesbeiana)

Abstract

1. The response patterns of single cells to monaural and binaural acoustic stimuli were studied in the dorsal medullary nucleus of the bullfrog (Rana catesbeiana). This nucleus represents the first ascending center in the anuran's central auditory nervous system. 2. Of the 142 cells isolated, 75 units responded only to monaural stimulation. Approximately 80% of these monaural cells could be excited by the ipsilateral ear, while the remaining 20% received their excitatory input from the contralateral ear. The other 67 units responded to binaural stimuli. Of these binaural cells, 14 could be excited by either contralateral or ipsilateral stimuli, and the threshold and best excitatory frequency were similar for each ear (EE). The other 53 binaural cells (EI) could be excited by stimulation of one ear and inhibited by stimulation of the other ear; for almost all of these cells the contralateral ear was excitatory and the ipsilateral ear was inhibitory. The best inhibitory frequency for one ear was approximately the same as the best excitatory frequency for the other ear, and the threshold for inhibition was near the threshold for excitation. 3. The tuning curves for all of the cells in the dorsal medullary nucleus were unimodal with "Q" values ranging from 0.4 to 4. The excitatory thresholds were widely scattered between 22 and 115 dB SPL. 4. The distribution of best excitatory frequencies for the monaural cells comprised three groups: 200-300, 500-800, and 900-1,600 Hz. The best excitatory frequencies of the binaural cells were scattered over this entire range, with a broad peak around 200-800 Hz. 5. Approximately 80% of the cells in the dorsal nucleus responded tonically throughout the duration of an excitatory tone burst. The remaining 20% of the cells responded phasically during the transient stages of a tone burst over a wide intensity range. 6. Response latencies were compared for the two types of monaural cells to tones at their best exciatatory frequencies at 10 dB above threshold. The latencies for the contralaterally excitable cells were just a few milliseconds longer than the latencies for the ipsilaterally excitable cells. For binaural cells the latency for contralateral stimulation was only 1-2 ms longer than for ipsilateral stimulation. It was concluded that the contralateral input to the dorsal medullary nucleus is not of efferent descending origin from higher auditory centers. 7. All of the binaural EI cells were sensitive to small interaural intensity differences and many were also sensitive to minute interaural time differences.These cells likely play a role in localization of sounds of significance to anurans.