Release of biologically active TGF-β from airway smooth muscle cells induces autocrine synthesis of collagen

Abstract

In severe or chronic asthma, there is an increase in airway smooth muscle cell (ASMC) mass as well as an increase in connective tissue proteins in the smooth muscle layer of airways. Transforming growth factor-β (TGF-β) exists in three isoforms in mammals and is a potent regulator of connective tissue protein synthesis. Using immunohistochemistry, we had previously demonstrated that ASMCs contain large quantities of TGF-β1–3. In this study, we demonstrate that bovine ASMC-derived TGF-β associates with the TGF-β latency binding protein-1 (LTBP-1) expressed by the same cells. The TGF-β associated with LTBP-1 localizes TGF-β extracellularly. Furthermore, plasmin, a serine protease, regulates the secretion of a biologically active form of TGF-β by ASMCs as well as the release of extracellular TGF-β. The biologically active TGF-β released by plasmin induces ASMCs to synthesize collagen I in an autocrine manner. The autocrine induction of collagen expression by ASMCs may contribute to the irreversible fibrosis and remodeling seen in the airways of some asthmatics.