Newly synthesized glycoprotein profiling to identify molecular signatures of warm ischemic injury in donor lungs-Reference-Cited by-同舟云学术

Newly synthesized glycoprotein profiling to identify molecular signatures of warm ischemic injury in donor lungs

Published:2023-07-01 Issue:1 Volume:325 Page:L30-L44
ISSN:1040-0605
Container-title:American Journal of Physiology-Lung Cellular and Molecular Physiology
language:en
Short-container-title:American Journal of Physiology-Lung Cellular and Molecular Physiology

Author:

Ling Zihan¹,Noda Kentaro²^ORCID,Frey Brian L.³^ORCID,Hu Michael¹^ORCID,Fok Shierly W.¹,Smith Lloyd M.³,Sanchez Pablo G.²,Ren Xi¹^ORCID

Affiliation:

1. Department of Biomedical Engineering, Carnegie Mellon University, Pittsburgh, Pennsylvania, United States

2. Division of Lung Transplant and Lung Failure, Department of Cardiothoracic Surgery, University of Pittsburgh, Pittsburgh, Pennsylvania, United States

3. Department of Chemistry, University of Wisconsin, Madison, Wisconsin, United States

Abstract

This study developed and implemented a bioorthogonal strategy to chemoselectively label, enrich, and characterize newly synthesized (NewS-)glycoproteins during 4-h ex vivo lung perfusion (EVLP). Through this approach, the investigators uncovered specific proteomic signatures associated with warm ischemic injury in donor lung grafts. These signatures exhibit high biological relevance to ischemia-reperfusion injury, validating the robustness of the presented approach.

Funder

China Scholarship Council

HHS | NIH | National Heart, Lung, and Blood Institute

Publisher

American Physiological Society

Subject

Cell Biology,Physiology (medical),Pulmonary and Respiratory Medicine,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajplung.00412.2022

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