Quantification of smooth muscle in human airways by polarization-sensitive optical coherence tomography requires correction for perichondrium

Author:

Hackmann Michael J.12ORCID,Cairncross Alvenia13ORCID,Elliot John G.13,Mulrennan Siobhain456,Nilsen Kris7ORCID,Thompson Bruce R.8,Li Qingyun2,Karnowski Karol29,Sampson David D.10ORCID,McLaughlin Robert A.21112ORCID,Cense Barry213,James Alan L.36,Noble Peter B.1ORCID

Affiliation:

1. School of Human Sciences, The University of Western Australia, Crawley, Western Australia, Australia

2. Department of Electrical, Electronic, and Computer Engineering, The University of Western Australia, Crawley, Western Australia, Australia

3. Department of Pulmonary Physiology and Sleep Medicine, Sir Charles Gairdner Hospital, Western Australia, Australia

4. Department of Respiratory Medicine, Sir Charles Gairdner Hospital, Perth, Western Australia, Australia

5. Institute of Respiratory Health, The University of Western Australia, Crawley, Western Australia, Australia

6. Medical School, The University of Western Australia, Crawley, Western Australia, Australia

7. Central Clinical School, Monash University, Melbourne, Victoria, Australia

8. Melbourne School of Health Sciences, The University of Melbourne, Melbourne, Victoria, Australia

9. International Centre for Translational Eye Research, Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland

10. School of Computer Science and Electronic Engineering, University of Surrey, Guildford, United Kingdom

11. Faculty of Health and Medical Sciences, The University of Adelaide, Adelaide, South Australia, Australia

12. Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, South Australia, Australia

13. Department of Mechanical Engineering, Yonsei University, Seoul, South Korea

Abstract

Polarization-sensitive optical coherence tomography (PS-OCT) may facilitate in vivo measurement of airway smooth muscle (ASM). We present a quantitative validation correlating absolute ASM area from PS-OCT to directly matched histological cross sections using human tissue. A major confounder for ASM quantification was observed and resolved: fibrous perichondrium surrounding hyaline cartilage in human airways presents a PS-OCT signature similar to ASM for birefringence and optic axis orientation. Findings impact the development of automated methods for ASM segmentation.

Funder

Future Health Research and Innovation Fund, Western Australia

Sir Charles Gairdner and Osborne Park Health Care Group Research Advisory Committee Project Grant

National Research Foundation of Korea

Polish Returns Program, Polish National Agency for Academic Exchange

Australian Government

University of Western Australia

DHAC | National Health and Medical Research Council

Department of Health, Australian Government | National Health and Medical Research Council

Publisher

American Physiological Society

Cited by 1 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Translating endobronchial optical coherence tomography to clinical practice;American Journal of Physiology-Lung Cellular and Molecular Physiology;2024-07-01

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