Time-resolved proteome profiling of normal lung development

Author:

Moghieb Ahmed1,Clair Geremy1,Mitchell Hugh D.1,Kitzmiller Joseph2,Zink Erika M.1,Kim Young-Mo1,Petyuk Vladislav1,Shukla Anil1,Moore Ronald J.1,Metz Thomas O.1,Carson James3,McDermott Jason E.1,Corley Richard A.1,Whitsett Jeffrey A.2,Ansong Charles1

Affiliation:

1. Biological Science Division, Pacific Northwest National Laboratory, Richland, Washington

2. Division of Pulmonary Biology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio

3. Texas Advanced Computing Center, University of Texas at Austin, Austin, Texas

Abstract

Biochemical networks mediating normal lung morphogenesis and function have important implications for ameliorating morbidity and mortality in premature infants. Although several transcript-level studies have examined normal lung development, corresponding protein-level analyses are lacking. Here we performed proteomics analysis of murine lungs from embryonic to early adult ages to identify the molecular networks mediating normal lung development. We identified 8,932 proteins, providing a deep and comprehensive view of the lung proteome. Analysis of the proteomics data revealed discrete modules and the underlying regulatory and signaling network modulating their expression during development. Our data support the cell proliferation that characterizes early lung development and highlight responses of the lung to exposure to a nonsterile oxygen-rich ambient environment and the important role of lipid (surfactant) metabolism in lung development. Comparison of dynamic regulation of proteomic and recent transcriptomic analyses identified biological processes under posttranscriptional control. Our study provides a unique proteomic resource for understanding normal lung formation and function and can be freely accessed at Lungmap.net.

Funder

HHS | NIH | National Heart, Lung, and Blood Institute (NHBLI)

Publisher

American Physiological Society

Subject

Cell Biology,Physiology (medical),Pulmonary and Respiratory Medicine,Physiology

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