Nitric oxide inhibits Na+absorption across cultured alveolar type II monolayers

Abstract

We examined the mechanisms by which nitric oxide (⋅ NO) decreased vectorial Na+ transport across confluent monolayers of rat alveolar type II (ATII) cells grown on permeable supports. Amiloride (10 μM) applied to the apical side of monolayers inhibited ∼90% of the equivalent ( I eq) and the short-circuit ( I sc) current, with an half-maximal inhibitory concentration (IC50) of 0.85 μM, indicating that Na+ entry into ATII cells occurred through amiloride-sensitive Na+ channels. ⋅ NO generated by spermine NONOate and papa NONOate added to both sides of the monolayers decreased I eq and increased transepithelial resistance in a concentration-dependent fashion (IC50 = 0.4 μM ⋅ NO). These changes were prevented or reversed by addition of oxyhemoglobin (50 μM). Incubation of ATII monolayers with 8-bromoguanosine 3′,5′-cyclic monophosphate (400 μM) had no effect on transepithelial Na+ transport. When the basolateral membranes of ATII cells were permeabilized with amphotericin B (10 μM) in the presence of a mucosal-to-serosal Na+ gradient (145:25 mM), ⋅ NO (generated by 100 μM papa NONOate) inhibited ∼60% of the amiloride-sensitive I sc. In addition, after permeabilization of the apical membranes, ⋅ NO inhibited the I sc[a measure of Na+-K+-adenosinetriphosphatase (ATPase) activity] by ∼60%. We concluded that ⋅ NO at noncytotoxic concentrations decreased Na+ absorption across cultured ATII monolayers by inhibiting both the amiloride-sensitive Na+ channels and Na+-K+-ATPase through guanosine 3′,5′-cyclic monophosphate-independent mechanisms.