Characteristics of Cl− uptake in rat alveolar type I cells

Author:

Johnson Meshell12,Allen Lennell3,Dobbs Leland134

Affiliation:

1. Department of Medicine,

2. San Francisco Veterans Affairs Medical Center, San Francisco, California

3. Cardiovascular Research Institute,

4. Department of Pediatrics, University of California, San Francisco, and

Abstract

Although Cl transport in fetal lung is important for fluid secretion and normal lung development, the role of Cl transport in adult lung is not well understood. In physiological studies, the cystic fibrosis transmembrane regulator (CFTR) plays a role in fluid absorption in the distal air spaces of adult lung, and alveolar type II cells cultured for 5 days have the capacity to transport Cl. Although both alveolar type I and type II cells express CFTR, it has previously not been known whether type I cells transport Cl. We studied Cl uptake in isolated type I cells directly, using either radioisotopic tracers or halide-sensitive fluorescent indicators. By both methods, type I cells take up Cl. In the presence of β-adrenergic agonist stimulation, Cl uptake can be inhibited by CFTR antagonists. Type I cells express both the Cl/HCO3 anion exchanger AE2 and the voltage-gated Cl channels CLC5 and CLC2. Inhibitors of AE2 also block Cl uptake in type I cells. Together, these results demonstrate that type I cells are capable of Cl uptake and suggest that the effects seen in whole lung studies establishing the importance of Cl movement in alveolar fluid clearance may be, in part, the result of Cl transport across type I cells.

Publisher

American Physiological Society

Subject

Cell Biology,Physiology (medical),Pulmonary and Respiratory Medicine,Physiology

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1. Is chlorine and sodium levels in the amniotic fluid a new marker for fetal lung maturation and RDS severity?;The Journal of Maternal-Fetal & Neonatal Medicine;2020-11-24

2. Ion channels of the lung and their role in disease pathogenesis;American Journal of Physiology-Lung Cellular and Molecular Physiology;2017-11-01

3. The role of stretch-activated ion channels in acute respiratory distress syndrome: finally a new target?;American Journal of Physiology-Lung Cellular and Molecular Physiology;2016-09-01

4. Alveolar Epithelium and Fluid Transport;Murray and Nadel's Textbook of Respiratory Medicine;2016

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