Multicompartmental analysis of the murine pulmonary immune response by spectral flow cytometry-Reference-Cited by-同舟云学术

Multicompartmental analysis of the murine pulmonary immune response by spectral flow cytometry

Published:2023-10-01 Issue:4 Volume:325 Page:L518-L535
ISSN:1040-0605
Container-title:American Journal of Physiology-Lung Cellular and Molecular Physiology
language:en
Short-container-title:American Journal of Physiology-Lung Cellular and Molecular Physiology

Author:

Chang Mary Y.¹²^ORCID,Brune Jourdan E.¹²^ORCID,Black Michele³^ORCID,Altemeier William A.²⁴^ORCID,Frevert Charles W.¹²⁴^ORCID

Affiliation:

1. Department of Comparative Medicine, University of Washington, Seattle, Washington, United States

2. Center for Lung Biology, University of Washington at South Lake Union, Seattle, Washington, United States

3. Department of Immunology, University of Washington, Seattle, Washington, United States

4. Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of Washington, Seattle, Washington, United States

Abstract

We describe a novel combination of methodologies that incorporates dual in vivo compartmental analysis using intravascular and intratracheal CD45 labeling, a broad panel of antibodies for identifying lymphoid and nonlymphoid cells, and spectral flow cytometry that uses cellular autofluorescence to aid in resolving and identifying immune cell populations. This methodology allows precise localization of immune cells in the lavageable airways, nonlavageable airways, interstitial lung tissue, and marginated in the lung vasculature.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Physiological Society

Subject

Cell Biology,Physiology (medical),Pulmonary and Respiratory Medicine,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajplung.00317.2022

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