Anoctamin-1 is induced by TGF-β and contributes to lung myofibroblast differentiation-Reference-Cited by-同舟云学术

Anoctamin-1 is induced by TGF-β and contributes to lung myofibroblast differentiation

Published:2024-01-01 Issue:1 Volume:326 Page:L111-L123
ISSN:1040-0605
Container-title:American Journal of Physiology-Lung Cellular and Molecular Physiology
language:en
Short-container-title:American Journal of Physiology-Lung Cellular and Molecular Physiology

Author:

Reed Eleanor B.¹,Orbeta Shaina²,Miao Bernadette A.¹,Sitikov Albert¹,Chen Bohao¹,Levitan Irena³⁴⁵,Solway Julian¹,Mutlu Gökhan M.¹^ORCID,Fang Yun¹,Mongin Alexander A.²^ORCID,Dulin Nickolai O.¹^ORCID

Affiliation:

1. Department of Medicine, Section of Pulmonary and Critical Care Medicine, The University of Chicago, Chicago, Illinois, United States

2. Department of Neuroscience and Experimental Therapeutics, Albany Medical College, Albany, New York, United States

3. Department of Medicine, University of Illinois at Chicago, Chicago, Illinois, United States

4. Department of Pharmacology, University of Illinois at Chicago, Chicago, Illinois, United States

5. Department of Bioengineering, University of Illinois at Chicago, Chicago, Illinois, United States

Abstract

This study describes a novel mechanism of differentiation of human lung fibroblasts (HLFs) to myofibroblasts: the key process in the pathogenesis of pulmonary fibrosis. Transforming growth factor-β (TGF-β) drives the expression of calcium-activated chloride channel anoctmin-1 (ANO1) leading to an increase in intracellular levels of chloride. The latter recruits chloride-sensitive with-no-lysine (K) kinase (WNK1) to activate profibrotic RhoA and AKT signaling pathways, possibly through activation of mammalian target of rapamycin complex-2 (mTORC2), altogether promoting myofibroblast differentiation.

Funder

HHS | NIH | National Institute of Neurological Disorders and Stroke

HHS | NIH | National Heart, Lung, and Blood Institute

Publisher

American Physiological Society

Subject

Cell Biology,Physiology (medical),Pulmonary and Respiratory Medicine,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajplung.00155.2023

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