Thermoregulatory vasomotor tone of the rat tail and paws in thermoneutral conditions and its impact on a behavioral model of acute pain

Abstract

The tail and paws in rodents are heat exchangers involved in the maintenance of core body temperature (Tcore). They are also the most widely used target organs to study acute or chronic “models” of pain. We describe the fluctuations of vasomotor tone in the tail and paws in conditions of thermal neutrality and the constraints of these physiological processes on the responses to thermal nociceptive stimuli, commonly used as an index of pain. Skin temperatures were recorded with a calibrated thermal camera to monitor changes of vasomotor tone in the tail and paws of awake and anesthetized rats. In thermoneutral conditions, the sympathetic tone fluctuated at a rate of two to seven cycles/h. Increased mean arterial blood pressure (MAP; ∼46 mmHg) was followed by increased heart rate (HR; ∼45 beats/min) within 30 s, vasoconstriction of extremities (3.5–7°C range) within 3–5 min, and increased Tcore (∼0.7°C) within 6 min. Decreased MAP was followed by opposite events. There was a high correlation between HR and Tcore recorded 5–6 min later. The reaction time of the animal's response to a radiant thermal stimulus—heat ramp (6°C/s, 20 mm2 spot) generated by a CO2 laser—directed to the tail depends on these variations. Consequently, the fluctuations in tail and paw temperature thus represent a serious confound for thermal nociceptive tests, particularly when they are conducted at thermal neutrality.