Inwardly rectifying currents of saccular hair cells from the leopard frog

Abstract

1. Inwardly rectifying currents were characterized in sensory hair cells isolated from the saccules of leopard frogs, using the whole cell configuration of the patch-clamp technique in voltage-clamp mode. 2. Two types of inwardly rectifying currents were distinguishable based on their ionic selectivity, activation and deactivation kinetics, voltage dependence, dependence on external K+ and sensitivity to divalent cations. 3. One inwardly rectifying current displayed K+ selectivity, rapid monoexponential activation (tau approximately 1 ms at -120 mV), steep voltage dependence, dependence of the activation voltage range on external K+ and block by external Ba2+. We refer to this current as IK1, consistent with the terminology used for a similar current in cardiac cells. In 5 mM external K+, IK1 activated negative to -60 mV, was half-activated at -86 mV and fully activated by -110 mV. 4. The other inwardly rectifying current was a mixed K+/Na+ current with slow sigmoidal activation (slow tau approximately 100 ms at -120 mV) and deactivation, shallow voltage dependence and no dependence of the activation curve on external K+ and which was blocked by external Cd2+. This current was called Ih because of its similarities to Ih of photoreceptors. Ih activated negative to -50 mV, was half-activated at -90 mV and was fully activated at -130 mV. 5. A correlation between cell shape and the type of inwardly rectifying current was noted; the more spherical cells had Ih alone and the more cylindrically shaped cells had Ih and IK1. 6. The mean resting potential of 115 cells with IK1 and Ih was -68 +/- 0.5 mV (mean +/- SE) and that of 53 cells with Ih alone was -50 +/- 0.5 mV. This suggests that IK1 contributes to the more negative resting potential of the cylindrical cells. 7. In current-clamp mode, the voltage responses to current steps of the two cell populations differed. Small negative current steps evoked faster, smaller responses in cells with IK1 and Ih than in cells with Ih alone. In cells with Ih alone, long (> 100 ms) negative current steps evoked a hyperpolarization that partly repolarized as Ih activated. Cells with Ih alone showed electrical resonance at rest whereas cells with IK1 resonated only in response to positive current steps. 8. A model developed to explain electrical resonance in bullfrog saccular hair cells was adapted to include Ih or IK1 and Ih.(ABSTRACT TRUNCATED AT 400 WORDS)