Electrophysiological Evidence for a Chemotopy of Biologically Relevant Odors in the Olfactory Bulb of the Channel Catfish

Abstract

Extracellular electrophysiological recordings from single olfactory bulb (OB) neurons in the channel catfish, Ictalurus punctatus, indicated that the OB is divided into different functional zones, each processing a specific class of biologically relevant odor. Different OB regions responded preferentially at slightly above threshold to either a mixture of 1) bile salts (10–7 to 10−5 M Na+ salts of taurocholic, lithocholic, and taurolithocholic acids), 2) nucleotides [10–6 to 10–4 M adenosine-5′-triphosphate (ATP), inosine-5′-monophosphate (IMP), and inosine-5′-triphosphate (ITP)], or 3) amino acids (10–6 to 10–4M l-alanine,l-methionine, l-arginine, andl-glutamate). Excitatory responses to bile salts were observed primarily in a thin, medial strip in both the dorsal (100–450 μm) and ventral (900–1,200 μm) OB. Excitatory responses to nucleotides were obtained primarily from dorsal, caudolateral OB, whereas excitatory responses to amino acids occurred more rostrally in the dorsolateral OB, but continued more medially in the ventral OB. The chemotopy within the channel catfish OB is more comparable to that previously described by optical imaging studies in zebrafish than by field potential studies in salmonids. The present results are consistent with recent studies, suggesting that the specific spatial organization of output neurons in the OB is necessary for the quality coding/decoding of olfactory information.