Chemogenetic activation of hypoglossal motoneurons in a mouse model of Pompe disease-Reference-Cited by-同舟云学术

Chemogenetic activation of hypoglossal motoneurons in a mouse model of Pompe disease

Published:2022-11-01 Issue:5 Volume:128 Page:1133-1142
ISSN:0022-3077
Container-title:Journal of Neurophysiology
language:en
Short-container-title:Journal of Neurophysiology

Author:

Singer Michele L.¹²³⁴^ORCID,Rana Sabhya²³⁴^ORCID,Benevides Ethan S.¹²³⁴^ORCID,Barral Brian E.³⁴,Byrne Barry J.⁵⁶,Fuller David D.¹²³⁴^ORCID

Affiliation:

1. Rehabilitation Science PhD Program, University of Florida, Gainesville, Florida

2. Department of Physical Therapy, University of Florida, Gainesville, Florida

3. Breathing Research and Therapeutics Center, University of Florida, Gainesville, Florida

4. McKnight Brain Institute, University of Florida, Gainesville, Florida

5. Department of Pediatrics, University of Florida, Gainesville, Florida

6. Powell Gene Therapy Center, University of Florida, Gainesville, Florida

Abstract

In a mouse model of Pompe disease, lingual injection of adeno-associated virus (AAV) serotype 9 encoding DREADD was histologically verified to produce transgene expression in hypoglossal motoneurons. Subsequent intraperitoneal delivery of a DREADD ligand stimulated tonic and phase tongue motor output.

Funder

HHS | NIH | Eunice Kennedy Shriver National Institute of Child Health and Human Development

HHS | NIH | NIH Office of the Director

University of Florida

Publisher

American Physiological Society

Subject

Physiology,General Neuroscience

Link

https://journals.physiology.org/doi/pdf/10.1152/jn.00026.2022

Reference40 articles.

1. Silencing of Hypoglossal Motoneurons Leads to Sleep Disordered Breathing in Lean Mice