Small hairpin inhibitory RNA delivery in the metanephric organ culture identifies long noncoding RNA <i>Pvt1</i> as a modulator of cyst growth-Reference-Cited by-同舟云学术

Small hairpin inhibitory RNA delivery in the metanephric organ culture identifies long noncoding RNA Pvt1 as a modulator of cyst growth

Published:2022-09-01 Issue:3 Volume:323 Page:F335-F348
ISSN:1931-857X
Container-title:American Journal of Physiology-Renal Physiology
language:en
Short-container-title:American Journal of Physiology-Renal Physiology

Author:

Eckberg Kara¹,Weisser Ivan¹,Buttram Daniel¹,Somia Nikunj²,Igarashi Peter¹^ORCID,Aboudehen Karam S.¹^ORCID

Affiliation:

1. Department of Medicine, University of Minnesota, Minneapolis, Minnesota

2. Department of Genetics, Cell Biology, and Development, University of Minnesota, Minneapolis, Minnesota

Abstract

This study identified scAAV/DJ as effective in transducing epithelial cells of the metanephric organ culture (MOC). We used scAAV/DJ shRNA to knockdown Pvt1 in cystic MOCs derived from Pkd1-null embryos. Downregulation of Pvt1 reduced cyst growth and decreased levels of c-MYC protein. These data suggest that suppression of Pvt1 activity in autosomal dominant polycystic kidney disease might reduce cyst growth.

Funder

HHS | NIH | National Institute of Diabetes and Digestive and Kidney Diseases

Publisher

American Physiological Society

Subject

Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajprenal.00016.2022

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5. Autosomal dominant polycystic kidney disease

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