Molecular characterization of human and rat organic anion transporter OATP-D

Author:

Adachi Hisanobu,Suzuki Takehiro,Abe Michiaki,Asano Naoki,Mizutamari Hiroya,Tanemoto Masayuki,Nishio Toshiyuki,Onogawa Tohru,Toyohara Takafumi,Kasai Satoshi,Satoh Fumitoshi,Suzuki Masanori,Tokui Taro,Unno Michiaki,Shimosegawa Tooru,Matsuno Seiki,Ito Sadayoshi,Abe Takaaki

Abstract

We have isolated and characterized a novel human and rat organic anion transporter subtype, OATP-D. The isolated cDNA from human brain encodes a polypeptide of 710 amino acids ( Mr 76,534) with 12 predicted transmembrane domains. The rat clone encodes 710 amino acids ( Mr 76,821) with 97.6% amino acid sequence homology with human OATP-D. Human and rat OATP-D have moderate amino acid sequence homology with LST-1/rlst-1, the rat oatp family, the prostaglandin transporter, and moat1/MOAT1/KIAA0880/OATP-B. Phylogenetic tree analysis revealed that OATP-D is branched in a different position from all known organic anion transporters. OATP-D transports prostaglandin E1 ( Km 48.5 nM), prostaglandin E2 ( Km 55.5 nM), and prostaglandin F, suggesting that, functionally, OATP-D encodes a protein that has similar characteristics to those of the prostaglandin transporter. Rat OATP-D also transports prostaglandins. The expression pattern of OATP-D mRNA was abundant mainly in the heart, testis, brain, and some cancer cells. Immunohistochemical analysis further revealed that rat OATP-D is widely expressed in the vascular, renal, and reproductive system at the protein level. These results suggest that OATP-D plays an important role in translocating prostaglandins in specialized tissues and cells.

Publisher

American Physiological Society

Subject

Physiology

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