A model-specific role of microRNA-223 as a mediator of kidney injury during experimental sepsis

Author:

Colbert James F.1ORCID,Ford Joshay A.1,Haeger Sarah M.1,Yang Yimu1,Dailey Kyrie L.1,Allison Kristen C.2,Neudecker Viola3,Evans Christopher M.1,Richardson Vanessa L.1,Brodsky Kelley S.2,Faubel Sarah1,Eltzschig Holger K.2,Schmidt Eric P.1,Ginde Adit A.4

Affiliation:

1. Department of Medicine, University of Colorado, Aurora, Colorado;

2. Department of Anesthesiology, University of Colorado, Aurora, Colorado;

3. Department of Anesthesiology, Clinic of the University of Munich, Munich, Germany; and

4. Department of Emergency Medicine, University of Colorado, Aurora, Colorado

Abstract

Sepsis outcomes are heavily dependent on the development of septic organ injury, but no interventions exist to interrupt or reverse this process. microRNA-223 (miR-223) is known to be involved in both inflammatory gene regulation and host-pathogen interactions key to the pathogenesis of sepsis. The goal of this study was to determine the role of miR-223 as a mediator of septic kidney injury. Using miR-223 knockout mice and multiple models of experimental sepsis, we found that miR-223 differentially influences acute kidney injury (AKI) based on the model used. In the absence of miR-223, mice demonstrated exaggerated AKI in sterile models of sepsis (LPS injection) and attenuated AKI in a live-infection model of sepsis (cecal ligation and puncture). We demonstrated that miR-223 expression is induced in kidney homogenate after cecal ligation and puncture, but not after LPS or fecal slurry injection. We investigated additional potential mechanistic explanations including differences in peritoneal bacterial clearance and host stool virulence. Our findings highlight the complex role of miR-223 in the pathogenesis of septic kidney injury, as well as the importance of differences in experimental sepsis models and their consequent translational applicability.

Funder

HHS | National Institutes of Health (NIH)

John A. Hartford Foundation

Publisher

American Physiological Society

Subject

Physiology

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