Eccentric contraction increases hydrogen peroxide levels and alters gene expression through Nox2 in skeletal muscle of male mice-Reference-Cited by-同舟云学术

Eccentric contraction increases hydrogen peroxide levels and alters gene expression through Nox2 in skeletal muscle of male mice

Published:2024-09-01 Issue:3 Volume:137 Page:778-788
ISSN:8750-7587
Container-title:Journal of Applied Physiology
language:en
Short-container-title:Journal of Applied Physiology

Author:

Kano Ryotaro¹²^ORCID,Kusano Tatsuya¹,Takeda Reo¹³,Shirakawa Hideki¹,Poole David C.⁴⁵^ORCID,Kano Yutaka¹⁶^ORCID,Hoshino Daisuke¹⁶^ORCID

Affiliation:

1. Department of Engineering Science, Bioscience and Technology Program, University of Electro-Communications, Chofu, Japan

2. Research Fellowship for Young Scientists, Japan Society for the Promotion of Science, Chiyoda, Japan

3. Cellular and Molecular Biotechnology Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

4. Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas, United States

5. Department of Kinesiology, Kansas State University, Manhattan, Kansas, United States

6. Center for Neuroscience and Biomedical Engineering (CNBE), University of Electro-Communications, Chofu, Japan

Abstract

This in vivo model successfully characterized the effects of eccentric (ECC) and concentric (CONC) contractions on cytosolic and mitochondrial [H2O2] in mouse skeletal muscle. Compared with CONC, ECC induced higher and more sustained [H2O2]cyto—an effect that was abolished by Nox2 inhibition. ECC-induced [H2O2]cyto elevations were requisite for altered gene expression.

Funder

MEXT | Japan Society for the Promotion of Science

Publisher

American Physiological Society

Link

https://journals.physiology.org/doi/pdf/10.1152/japplphysiol.00335.2024

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