Affiliation:
1. Department of Medicine, Hinchingbrooke Hospital, Huntingdon;
2. Papworth Hospital, Cambridge, United Kingdom; and
3. Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, Texas
Abstract
Lung diffusing capacity for nitric oxide (DlNO) is used to measure alveolar membrane conductance (DmNO), but disagreement remains as to whether DmNO = DlNO, and whether blood conductance (θNO) = ∞. Our previous in vitro and in vivo studies suggested that θNO < ∞. We now show in a membrane oxygenator model perfused with whole blood that addition of a cell-free bovine hemoglobin (Hb) glutamer-200 solution increased diffusing capacity of the circuit (D) for NO (Dno) by 39%, D for carbon monoxide (Dco) by 24%, and the ratio of Dno to Dco by 12% (all P < 0.001). In three anesthetized dogs, DlNO and DlCO were measured by a rebreathing technique before and after three successive equal volume-exchange transfusions with bovine Hb glutamer-200 (10 ml/kg each, total exchange 30 ml/kg). At baseline, DlNO/DlCO = 4.5. After exchange transfusion, DlNO rose 57 ± 16% (mean ± SD, P = 0.02) and DlNO/DlCO = 7.1, whereas DlCO remained unchanged. Thus, in vitro and in vivo data directly demonstrate a finite θNO. We conclude that the erythrocyte and/or its immediate environment imposes considerable resistance to alveolar-capillary NO uptake. DlNO is sensitive to dynamic hematological factors and is not a pure index of conductance of the alveolar tissue membrane. With successive exchange transfusion, the estimated in vivo θNO [5.1 ml NO·(ml blood·min·Torr)−1] approached 4.5 ml NO·(ml blood·min·Torr)−1, which was derived from in vitro measurements by Carlsen and Comroe ( J Gen Physiol 42: 83–107, 1958). Therefore, we suggest use of θNO = 4.5 ml NO·(min·Torr·ml blood)−1 for calculation of DmNO and pulmonary capillary blood volume from DlNO and DlCO.
Publisher
American Physiological Society
Subject
Physiology (medical),Physiology
Cited by
64 articles.
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