Lactate metabolism in resting and contracting canine skeletal muscle with elevated lactate concentration

Abstract

This study was undertaken to quantitatively account for the metabolic disposal of lactate in skeletal muscle exposed to an elevated lactate concentration during rest and mild-intensity contractions. The gastrocnemius plantaris muscle group (GP) was isolated in situ in seven anesthetized dogs. In two experiments, the muscles were perfused with an artificial perfusate with a blood lactate concentration of ∼9 mM while normal blood gas/pH status was maintained with [U-14C]lactate included to follow lactate metabolism. Lactate uptake and metabolic disposal were measured during two consecutive 40-min periods, during which the muscles rested or contracted at 1.25 Hz. Oxygen consumption averaged 10.1 ± 2.0 μmol · 100 g−1 · min−1 (2.26 ± 0.45 ml · kg−1 · min−1) at rest and 143.3 ± 16.2 μmol · 100 g−1 · min−1 (32.1 ± 3.63 ml · kg−1 · min−1) during contractions. Lactate uptake was positive during both conditions, increasing from 10.5 μmol · 100 g−1 · min−1 at rest to 25.0 μmol · 100 g−1 · min−1during contractions. Oxidation and glycogen synthesis represented minor pathways for lactate disposal during rest at only 6 and 15%, respectively, of the [14C]lactate removed by the muscle. The majority of the [14C]lactate removed by the muscle at rest was recovered in the muscle extracts, suggesting that quiescent muscle serves as a site of passive storage for lactate carbon during high-lactate conditions. During contractions, oxidation was the dominant means for lactate disposal at >80% of the [14C]lactate removed by the muscle. These results suggest that oxidation is a limited means for lactate disposal in resting canine GP exposed to elevated lactate concentrations due to the muscle's low resting metabolic rate.