Heat stress ameliorates tenotomy-induced inflammation in muscle-specific response via regulation of macrophage subtypes

Abstract

During disuse-induced muscle atrophy, macrophages play a significant role in inflammatory responses that occur with muscle degeneration and repair. Heat treatment has been shown to alleviate muscle atrophy; however, the effect of heat on inflammatory responses following tenotomy has not been evaluated. This study examined the effects of heat stress on proinflammatory (M1-like) and anti-inflammatory (M2-like) macrophage populations. Also, cytokine protein expression in oxidative soleus and glycolytic plantaris muscles following Achilles tendon transection (tenotomy) was analyzed. Male Wistar rats were assigned into control, control plus heat stress, tenotomy, and tenotomy plus heat stress groups. Tenotomy was performed for 8 (TEN8) and 14 (TEN14) days to induce muscle inflammation. Heat treatments, 30 min at 40.5–41.5°C, were given 24 h before and 1–6 consecutive days after tenotomy (TEN8 group) or every other day (TEN14 group). Tenotomy induced muscle necrosis, extensive infiltration of M1- (CD68+), and M2- (CD163+) like macrophages and increased tumor necrosis factor-α (TNFα) but not interleukin-10 (IL-10) protein expression. Heat stress caused a reduction in necrotic fibers, M1-like macrophage invasion, and TNFα protein expression in tenotomized soleus muscle. Additionally, heat stress enhanced M2-like macrophage accumulation during the 14 days following tenotomy in soleus muscle but did not affect IL-10 protein level. Our results indicate that heat stress can limit tenotomy-induced inflammatory responses through modulation of macrophage subtypes and TNFα protein expression, preferentially in oxidative muscle. These findings shed light on the ability of heat stress as a therapeutic strategy to manipulate macrophages for optimal inflammation during muscle atrophy. NEW & NOTEWORTHY We investigated differential effects of heat stress on modulating inflammation following 8 and 14 days of tenotomy in soleus and plantaris muscles. Heat exposure could reduce necrosis, suppress pro-inflammatory macrophage infiltration, and diminish TNFα protein expression in tenotomized muscle, which preferentially occurred in soleus muscle. Additionally, heat stress enhanced anti-inflammatory macrophages in soleus muscle in the 14-day study period. Neither tenotomy nor heat stress had an impact on IL-10 protein expression in either muscle examined.