High-frequency speckle tracking echocardiography in the assessment of left ventricular function and remodeling after murine myocardial infarction

Abstract

The objectives of this study were to assess the feasibility and accuracy of high-frequency speckle tracking echocardiography (STE) in a murine model of myocardial infarction (MI). STE is used clinically to quantify global and regional cardiac function, but its application in mice is challenging because of the small cardiac size and rapid heart rates. A high-frequency microultrasound system with STE (Visualsonics Vevo 2100) was compared against magnetic resonance imaging (MRI) for the assessment of global left ventricular (LV) size and function after murine MI. Animals subjected to coronary ligation ( n = 46) or sham ligation ( n = 27) were studied 4 wk postoperatively. Regional and global deformation were also assessed. STE-derived LV ejection fraction (EF) and mass correlated well with MRI indexes ( r = 0.93, 0.77, respectively; P < 0.001), as did STE-derived mass with postmortem values ( r = 0.80, P < 0.001). Higher STE-derived volumes correlated positively with MRI-derived infarct size ( P < 0.01). Global strain parameters were significantly reduced after MI (all P < 0.001) and strongly correlated with LV mass and MRI-derived infarct size as promising surrogates for the extent of remodeling and infarction, respectively (both P < 0.05). Regional strain analyses showed that radial strain and strain rate were relatively preserved in anterior basal segments after MI compared with more apical segments ( P < 0.001); however, longitudinal strain and strain rate were significantly impaired both basally and distally ( P < 0.001). Strain-derived parameters of dyssynchrony were significantly increased in the MI group ( P < 0.01). Analysis time for STE was 210 ± 45 s with acceptable inter- and intraobserver variability. In conclusion, high-frequency STE enables quantitative assessment of regional and global function in the remodeling murine LV after MI.