Affiliation:
1. Department of Cellular and Integrative PhysiologyUniversity of Nebraska Medical CenterOmaha, Nebraska, United States
Abstract
We have developed standardized protocols that significantly reduce cytotoxicity and enhance transfection efficiency in H9c2 and HL-1 cardiomyocyte cell lines. Our detailed comparative analysis of polymer-based and lipid-based transfection methods has identified optimized approaches with superior performance. Accompanying these protocols are comprehensive troubleshooting strategies to address common issues related to low transfection rates. Implementing these protocols is expected to yield more consistent and reproducible results, driving the field of cardiovascular research toward impactful clinical breakthroughs.
Funder
HHS | National Institutes of Health
UNMC | Department of Internal Medicine, University of Nebraska Medical Center
University of Nebraska Omaha
Publisher
American Physiological Society