Demonstration of age-related blood-brain barrier disruption and cerebromicrovascular rarefaction in mice by longitudinal intravital two-photon microscopy and optical coherence tomography

Author:

Nyúl-Tóth Ádám12,Tarantini Stefano134,DelFavero Jordan1,Yan Feng5,Balasubramanian Priya1ORCID,Yabluchanskiy Andriy1ORCID,Ahire Chetan1,Kiss Tamas16,Csipo Tamas13ORCID,Lipecz Agnes13,Farkas Attila E.27ORCID,Wilhelm Imola28ORCID,Krizbai István A.28,Tang Qinggong5,Csiszar Anna136,Ungvari Zoltan1346

Affiliation:

1. Vascular Cognitive Impairment and Neurodegeneration Program, Oklahoma Center For Geroscience and Healthy Aging, Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma

2. International Training Program in Geroscience, Institute of Biophysics, Biological Research Centre, Eötvös Loránd Research Network, Szeged, Hungary

3. International Training Program in Geroscience, Doctoral School of Basic and Translational Medicine/Department of Public Health, Semmelweis University, Budapest, Hungary

4. Department of Health Promotion Sciences, College of Public Health, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma

5. Stephenson School of Biomedical Engineering, Gallogly College of Engineering, The University of Oklahoma, Norman, Oklahoma

6. International Training Program in Geroscience, Theoretical Medicine Doctoral School/Departments of Medical Physics and Informatics and Cell Biology and Molecular Medicine, University of Szeged, Szeged, Hungary

7. Department of Physiology, Anatomy and Neuroscience, University of Szeged, Szeged, Hungary

8. Institute of Life Sciences, Vasile Goldiş Western University of Arad, Arad, Romania

Abstract

Methods are presented for longitudinal detection of age-related increase in blood-brain barrier permeability and microvascular rarefaction in the mouse cerebral cortex by intravital two-photon microscopy and optical coherence tomography.

Funder

HHS | NIH | National Cancer Institute

HHS | NIH | National Institute of General Medical Sciences

HHS | NIH | National Institute of Neurological Disorders and Stroke

HHS | NIH | National Institute on Aging

Publisher

American Physiological Society

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

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