SERCA2a-phospholamban interaction monitored by an interposed circularly permutated green fluorescent protein-Reference-Cited by-同舟云学术

SERCA2a-phospholamban interaction monitored by an interposed circularly permutated green fluorescent protein

Published:2021-06-01 Issue:6 Volume:320 Page:H2188-H2200
ISSN:0363-6135
Container-title:American Journal of Physiology-Heart and Circulatory Physiology
language:en
Short-container-title:American Journal of Physiology-Heart and Circulatory Physiology

Author:

Arnold Maren E.¹²^ORCID,Dostmann Wolfgang R.³,Martin Jody⁴,Previs Michael J.¹,Palmer Bradley¹,LeWinter Martin¹^ORCID,Meyer Markus⁵

Affiliation:

1. Department of Medicine and Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine, Burlington, Vermont

2. Institute of Experimental and Clinical Pharmacology und Toxicology, Faculty of Medicine, Albert-Ludwigs-University Freiburg, Freiburg, Germany

3. Department of Pharmacology, University of Vermont Larner College of Medicine, Burlington, Vermont

4. Department of Pharmacology, School of Medicine, Cardiovascular Research Institute, University of California, Davis, California

5. Department of Medicine, Lillehei Heart Institute, University of Minnesota College of Medicine, Minneapolis, Minnesota

Abstract

This study describes the design and characterization of a novel biosensor that can visualize the interaction of SERCA2a and phospholamban (PLB). The biosensor combines SERCA2a, a circularly permutated green fluorescent protein, and PLB into one recombinant protein (SGP). Proteinkinase A activation results in phosphorylation of the PLB domain and is associated with a marked increase in the fluorescence yield to allow for real-time monitoring of the SERCA2a and PLB interaction in cells.

Funder

Totman Trust

HHS | National Institutes of Health

Publisher

American Physiological Society

Subject

Physiology (medical),Cardiology and Cardiovascular Medicine,Physiology

Link

https://journals.physiology.org/doi/pdf/10.1152/ajpheart.00858.2020

Reference49 articles.

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5. Targeted ablation of the phospholamban gene is associated with markedly enhanced myocardial contractility and loss of beta-agonist stimulation.

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